Study of silanized nanostructures with immobilized fumarase for production of L-malate

The conversion of fumaric acid into L-malate by fumarase immobilized on silanized nanostructures was analyzed experimentally. enzyme bound to the nanostructures. We carried out scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) analysis, zeta size analysis and surface area calculation for characterization effect initial concentration pH immobilization procedure were investigated change Michaelis-Menten constants (Km Vmax) with examined. in storage stability also investigated. very good. observed that [p(HEMA)-3-MTES] much greater amounts. have compared activities free we a significant increase activity after production. Moreover, came conclusion this can be better preserved 30 days fumarase.